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KMID : 0358920070340020299
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Journal of the Korean Academy of Pedodontics
2007 Volume.34 No. 2 p.299 ~ p.308
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THE EFFECTS OF SUGARS ON THE EXPRESSION OF GTFB AND GTFC MRNA
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Chung Hye-Jin
Kim Shin
Chung Jin
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Abstract
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Insoluble glucan is the important component of oral biofilm, which is synthesized from sucrose through the action of glucosyltransferase (GTF) B and GTF C encoded by the gtfB and gtfC genes, respectively of Streptococcus mutans. In present study, the effects of various sugars on the mRNA expression of gtfB and gtfC of S. mutans Ingbritt were examined by fluorescent in situ hybridization (FISH). The mRNA of gtfB and gtfC was expressed normally in the BHI broth containing 1% and 5% sucrose. The mRNA expression was decreased by the addition of 10% of glucose, and 1%, 5% and 10% of fructose. Lactose had no great effect on the expression of gtfB and gtfC. 5% and 10% of xylitol greatly reduced the mRNA expression of gtfB and gtfC. Sorbitol slightly decreased the mRNA expression of gtfB and gtfC when compared to the control. In summary, mRNA expression of gtfB and gtfC was decreased by the addition of glucose, fructose, and xylitol.
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KEYWORD
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Streptococcus mutans, Glucosyltransferase, Fluorescent in situ hybridization, Xylitol, Sorbitol
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